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polA [2019-01-30 09:29:11]
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polA [2019-01-30 09:29:11]

DNA polymerase I, required for repair of UV- or MMS-induced DNA damage, processing of Okazaki fragments
locus
BSU29090
pI
4.92
mw
98.89 kDa
protein length
880 aa Sequence Blast
gene length
2643 bp Sequence Blast
function
DNA replication
product
DNA polymerase I
essential
no
ec
2.7.7.7
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    2,973,182 2,975,824

    Phenotypes of a mutant

  • strongly reduced stationary phase mutagenesis [Pubmed|27399782]
  • a [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA] [gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] double mutant is not viable (synthetic lethality) [pubmed|17114254]
  • a [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC] [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA] double mutant is not viable at 25°C [pubmed|30670546]

    • The protein

    Catalyzed reaction/ biological activity

  • has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity [Pubmed|16045613]
  • maturation of Okazaki fragments (together with [protein|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|RNase HIII]) [pubmed|30670546]

    • Protein family

  • DNA polymerase type-A family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|ExoR]:

    • Structure

  • [PDB|1XWL] (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)

    • Expression and Regulation

    Operons

    genes
    [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]-[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]-[gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]-[gene|E163A282560DB882B81E783E7E95E1960F4AE4D2|ytaG]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|23396918], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
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    Biological materials

    Mutant

  • BKE29090 ([gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
  • BKK29090 ([gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA

    • References

  • 17114254,16045613,17905985,27399782,30670546